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Literature summary extracted from

  • Boehmer, P.E.; Emmerson, P.T.
    Escherichia coli RecBCD enzyme: inducible overproduction and reconstitution of the ATP-dependent deoxyribonuclease from purified subunits (1991), Gene, 102, 1-6.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.11.5 fusion of the recBCD genes and expression in Escherichia coli Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.5 65000
-
 1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli
3.1.11.5 130000
-
 1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli
3.1.11.5 140000
-
 1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.5 of the recombinant proteins B, C and D Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 double-stranded DNA + H2O no exonuclease activity in recD null mutants Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.5 trimer 1 * 140000 + 1 * 130000 + 1 * 65000, SDS-PAGE Escherichia coli